Sperm cryopreservation is a procedure to preserve sperm cells. Sperms can be used successfully indefinitely after cryopreservation. For human sperm, the longest reported successful storage is 21 years. It can be used for sperm donation where the recipient wants the treatment in a different time or place to have children.
Methodology for Processing, Freezing and Storing Sperm Samples
- Sperm banking is performed by first analyzing your semen to determine the sperm count and motility of the sperm.
- Each sample (ejaculate) is then mixed with a special media or solution to help provide protection during freezing and thawing.
- The samples are then placed in high security CBS™ cryobiostraws which are coded and carefully frozen by liquid nitrogen vapour.
- The straws are immersed in liquid nitrogen and are stored in liquid nitrogen storage tanks at a temperature of -196° C (-371° F). At these low temperatures, any biological activity, including the biochemical reactions that would lead to cell death, is effectively stopped.
- The liquid nitrogen is independent of any source of power, and is regularly checked and replenished.
- The CBS™ cryobiostraw is designed for the cryo-storage of biological samples of human origin.
- The physical properties of the Cryobiostraw and the method of filling and sealing provide a leak proof environment for the specimen even when immersed in liquid nitrogen to avoid contamination with the infected samples. Manufactured with biocompatible materials, the CBS™ straw is ideal for use in Assisted Reproduction Technologies, particularly for cryopreservation of sperm and embryos.